Immobilization and Culturing of Mammalian Cells with Bio- Compatible Electrodeposition of Calcium Alginate Gel in Microfluidic Devices
نویسندگان
چکیده
This paper reports a unique approach to immobilize mammalian cell populations (mouse myeloma NS0) through in situ gelation of calcium alginate triggered by an electrical signal under physiologically relevant conditions. This is the first experimental observation to evaluate the electrically triggered assembly of calcium alginate gel for entrapping and culturing mammalian cells. Subsequent cell viabilities immediately after electrodeposition and after three days of culturing are studied. Our cell assembly strategy is applicable to fragile mammalian cells and can be used for in vitro study of dynamic cellular processes and cell-based assays under a microfluidic environment.
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